Biology MCQs

Molecular Medicine MCQs with Answers

Which of the following is a technique used to amplify specific DNA sequences?
a) PCR
b) ELISA
c) Western blotting
d) Northern blotting
Answer: a) PCR

What is the term for a section of DNA that codes for a specific protein?
a) Gene
b) Allele
c) Chromosome
d) Genome
Answer: a) Gene

Which of the following is a genetic disorder caused by a mutation in the CFTR gene?
a) Parkinson’s disease
b) Cystic fibrosis
c) Alzheimer’s disease
d) Hemophilia
Answer: b) Cystic fibrosis

What is the term for the complete set of genes or genetic material present in a cell or organism?
a) Gene
b) Allele
c) Chromosome
d) Genome
Answer: d) Genome

Which of the following is a technique used to separate DNA fragments based on size using an electric field?
a) PCR
b) ELISA
c) Gel electrophoresis
d) Southern blotting
Answer: c) Gel electrophoresis

What is the term for a change in the DNA sequence that can be inherited?
a) Mutation
b) Polymorphism
c) Allele
d) Genotype
Answer: a) Mutation

Which of the following is a technique used to detect and quantify specific proteins in a sample?
a) PCR
b) ELISA
c) Western blotting
d) Northern blotting
Answer: c) Western blotting

What is the term for a specific version of a gene?
a) Gene
b) Allele
c) Chromosome
d) Genome
Answer: b) Allele

Which of the following is a genetic disorder caused by an abnormality in the number of sex chromosomes?
a) Down syndrome
b) Turner syndrome
c) Sickle cell anemia
d) Huntington’s disease
Answer: b) Turner syndrome

What is the term for the process of making an RNA molecule from a DNA template?
a) Transcription
b) Translation
c) Replication
d) Transformation
Answer: a) Transcription

Which of the following is a technique used to determine the order of nucleotides in a DNA molecule?
a) PCR
b) ELISA
c) Sanger sequencing
d) Western blotting
Answer: c) Sanger sequencing

What is the term for the process of decoding the information in an mRNA molecule to build a protein?
a) Transcription
b) Translation
c) Replication
d) Transformation
Answer: b) Translation

Which of the following is a genetic disorder caused by a mutation in the HTT gene?
a) Parkinson’s disease
b) Cystic fibrosis
c) Huntington’s disease
d) Hemophilia
Answer: c) Huntington’s disease

What is the term for a diagram that shows the inheritance pattern of a trait or genetic disorder in a family?
a) Punnett square
b) Pedigree
c) Genotype
d) Phenotype
Answer: b) Pedigree

Which of the following is a technique used to detect and quantify gene expression levels?
a) PCR
b) ELISA
c) Microarray
d) Northern blotting
Answer: c) Microarray

What is the term for a change in the DNA sequence that does not result in a change in the amino acid sequence of a protein?
a) Mutation
b) Polymorphism
c) Allele
d) Genotype
Answer: b) Polymorphism

Which of the following is a technique used to introduce foreign DNA into a host organism?
a) PCR
b) ELISA
c) Cloning
d) Transformation
Answer: d) Transformation

What is the term for a specific combination of alleles in an individual’s genome?
a) Gene
b) Allele
c) Genotype
d) Phenotype
Answer: c) Genotype

Which of the following is a genetic disorder caused by a mutation in the SMN1 gene?
a) Spinal muscular atrophy
b) Down syndrome
c) Sickle cell anemia
d) Parkinson’s disease
Answer: a) Spinal muscular atrophy

What is the term for a technique used to visualize and study the structure and function of molecules?
a) PCR
b) ELISA
c) X-ray crystallography
d) Northern blotting
Answer: c) X-ray crystallography

Which of the following is a technique used to analyze gene expression patterns in a sample?
a) PCR
b) ELISA
c) Microarray
d) Western blotting
Answer: c) Microarray

What is the term for the observable characteristics of an individual resulting from the interaction of genes and the environment?
a) Gene
b) Allele
c) Genotype
d) Phenotype
Answer: d) Phenotype

Which of the following is a technique used to detect and quantify specific RNA molecules in a sample?
a) PCR
b) ELISA
c) Northern blotting
d) Western blotting
Answer: c) Northern blotting

What is the term for a genetic disorder caused by the presence of an extra copy of chromosome 21?
a) Down syndrome
b) Turner syndrome
c) Sickle cell anemia
d) Huntington’s disease
Answer: a) Down syndrome

Which of the following is a technique used to determine the presence and quantity of a specific DNA or RNA sequence?
a) PCR
b) ELISA
c) Southern blotting
d) Western blotting
Answer: c) Southern blotting

What is the term for a genetic disorder caused by a mutation in the FMR1 gene?
a) Fragile X syndrome
b) Cystic fibrosis
c) Alzheimer’s disease
d) Hemophilia
Answer: a) Fragile X syndrome

Which of the following is a technique used to study protein-protein interactions?
a) PCR
b) ELISA
c) Co-immunoprecipitation
d) Western blotting
Answer: c) Co-immunoprecipitation

What is the term for a technique used to determine the presence or absence of a specific DNA sequence in a sample?
a) PCR
b) ELISA
c) Polymerase chain reaction
d) Transformation
Answer: a) PCR

Which of the following is a technique used to analyze gene expression levels in a high-throughput manner?
a) PCR
b) ELISA
c) Microarray
d) Northern blotting
Answer: c) Microarray

What is the term for the process of making multiple copies of a specific DNA sequence in vitro?
a) PCR
b) ELISA
c) Cloning
d) Transformation
Answer: a) PCR

Which of the following is a technique used to study gene expression at the single-cell level?
a) PCR
b) ELISA
c) Single-cell RNA sequencing
d) Western blotting
Answer: c) Single-cell RNA sequencing

What is the term for a genetic disorder caused by a mutation in the BRCA1 or BRCA2 gene?
a) Breast cancer
b) Cystic fibrosis
c) Alzheimer’s disease
d) Hemophilia
Answer: a) Breast cancer

Which of the following is a technique used to study the three-dimensional structure of proteins?
a) PCR
b) ELISA
c) X-ray crystallography
d) Northern blotting
Answer: c) X-ray crystallography

What is the term for a technique used to determine the presence and quantity of specific proteins in a sample?
a) PCR
b) ELISA
c) Western blotting
d) Southern blotting
Answer: c) Western blotting

Which of the following is a technique used to study gene function by selectively inactivating or modifying genes?
a) PCR
b) ELISA
c) RNA interference
d) Transformation
Answer: c) RNA interference

What is the term for a genetic disorder caused by a mutation in the SMN1 gene?
a) Spinal muscular atrophy
b) Down syndrome
c) Sickle cell anemia
d) Parkinson’s disease
Answer: a) Spinal muscular atrophy

Which of the following is a technique used to analyze the expression of all genes in a genome simultaneously?
a) PCR
b) ELISA
c) Microarray
d) Northern blotting
Answer: c) Microarray

What is the term for a technique used to determine the sequence of nucleotides in a DNA or RNA molecule?
a) PCR
b) ELISA
c) Sequencing
d) Transformation
Answer: c) Sequencing

Which of the following is a technique used to study protein-DNA interactions?
a) PCR
b) ELISA
c) Chromatin immunoprecipitation
d) Western blotting
Answer: c) Chromatin immunoprecipitation

What is the term for a technique used to introduce specific changes into the DNA sequence of a gene?
a) PCR
b) ELISA
c) Gene editing
d) Transformation
Answer: c) Gene editing

Which of the following is a technique used to determine the presence and quantity of specific RNA molecules in a sample?
a) PCR
b) ELISA
c) Northern blotting
d) Western blotting
Answer: c) Northern blotting

What is the term for a genetic disorder caused by a mutation in the ATP7B gene?
a) Wilson’s disease
b) Cystic fibrosis
c) Alzheimer’s disease
d) Hemophilia
Answer: a) Wilson’s disease

Which of the following is a technique used to study the function of specific genes by selectively activating or modifying them?
a) PCR
b) ELISA
c) Gene knockout
d) Transformation
Answer: c) Gene knockout

What is the term for a technique used to determine the presence and quantity of specific proteins in a sample?
a) PCR
b) ELISA
c) Western blotting
d) Southern blotting
Answer: c) Western blotting

Which of the following is a technique used to study the interactions between proteins and small molecules?
a) PCR
b) ELISA
c) Protein-protein interaction assays
d) Western blotting
Answer: c) Protein-protein interaction assays

What is the term for a genetic disorder caused by a mutation in the DMD gene?
a) Duchenne muscular dystrophy
b) Down syndrome
c) Sickle cell anemia
d) Huntington’s disease
Answer: a) Duchenne muscular dystrophy

Which of the following is a technique used to study the function and expression of all genes in a genome?
a) PCR
b) ELISA
c) High-throughput sequencing
d) Northern blotting
Answer: c) High-throughput sequencing

What is the term for a technique used to determine the presence and quantity of specific DNA or RNA sequences using fluorescent probes?
a) PCR
b) ELISA
c) Fluorescent in situ hybridization
d) Transformation
Answer: c) Fluorescent in situ hybridization

Which of the following is a technique used to study the function and interaction of proteins in a high-throughput manner?
a) PCR
b) ELISA
c) Proteomics
d) Western blotting
Answer: c) Proteomics

What is the term for a technique used to determine the presence and quantity of specific proteins in a sample based on their antigen-antibody interactions?
a) PCR
b) ELISA
c) Western blotting
d) Southern blotting
Answer: b) ELISA

Which of the following is a technique used to study the interactions between proteins and DNA?
a) PCR
b) ELISA
c) Electrophoretic mobility shift assay
d) Western blotting
Answer: c) Electrophoretic mobility shift assay

What is the term for a technique used to study the expression of specific genes in different tissues or under different conditions?
a) PCR
b) ELISA
c) RT-PCR
d) Northern blotting
Answer: c) RT-PCR

Which of the following is a technique used to study the function and expression of genes on a genome-wide scale?
a) PCR
b) ELISA
c) Genomics
d) Northern blotting
Answer: c) Genomics

What is the term for a technique used to determine the presence and quantity of specific DNA or RNA sequences using fluorescently-labeled primers?
a) PCR
b) ELISA
c) Real-time PCR
d) Transformation
Answer: c) Real-time PCR

Which of the following is a technique used to study the expression of all genes in a genome at a specific time point?
a) PCR
b) ELISA
c) RNA sequencing
d) Western blotting
Answer: c) RNA sequencing

What is the term for a technique used to determine the presence and quantity of specific proteins in a sample based on their size and charge?
a) PCR
b) ELISA
c) SDS-PAGE
d) Southern blotting
Answer: c) SDS-PAGE

Which of the following is a technique used to study the expression of specific genes by labeling and visualizing their RNA transcripts?
a) PCR
b) ELISA
c) In situ hybridization
d) Western blotting
Answer: c) In situ hybridization

What is the term for a technique used to determine the presence and quantity of specific proteins in a sample based on their migration in an electric field?
a) PCR
b) ELISA
c) SDS-PAGE
d) Northern blotting
Answer: c) SDS-PAGE

Which of the following is a technique used to study the function and expression of all proteins in a cell or organism?
a) PCR
b) ELISA
c) Proteomics
d) Western blotting
Answer: c) Proteomics

What is the term for a technique used to determine the presence and quantity of specific DNA or RNA sequences using radioactive probes?
a) PCR
b) ELISA
c) Radioactive labeling
d) Transformation
Answer: c) Radioactive labeling

Which of the following is a technique used to study the function and interaction of proteins in a high-throughput manner?
a) PCR
b) ELISA
c) Proteomics
d) Western blotting
Answer: c) Proteomics

What is the term for a technique used to determine the presence and quantity of specific proteins in a sample based on their antigen-antibody interactions using a fluorescently-labeled secondary antibody?
a) PCR
b) ELISA
c) Fluorescent antibody labeling
d) Southern blotting
Answer: c) Fluorescent antibody labeling

Which of the following is a technique used to study the interactions between proteins and RNA?
a) PCR
b) ELISA
c) RNA immunoprecipitation
d) Western blotting
Answer: c) RNA immunoprecipitation

What is the term for a technique used to determine the presence and quantity of specific DNA or RNA sequences using fluorescently-labeled nucleotide analogs?
a) PCR
b) ELISA
c) Fluorescent in situ hybridization
d) Transformation
Answer: c) Fluorescent in situ hybridization

Which of the following is a technique used to study the expression of all genes in a genome simultaneously based on the principle of hybridization?
a) PCR
b) ELISA
c) Microarray
d) Northern blotting
Answer: c) Microarray

What is the term for a technique used to determine the presence and quantity of specific proteins in a sample based on their migration in a gel under the influence of an electric field?
a) PCR
b) ELISA
c) SDS-PAGE
d) Western blotting
Answer: c) SDS-PAGE

Which of the following is a technique used to study the function and expression of specific genes by introducing fluorescently-labeled nucleotides during the synthesis of cDNA?
a) PCR
b) ELISA
c) Fluorescent labeling of cDNA
d) Transformation
Answer: c) Fluorescent labeling of cDNA

What is the term for a technique used to determine the presence and quantity of specific DNA or RNA sequences using a labeled probe that binds to the target sequence?
a) PCR
b) ELISA
c) Hybridization
d) Transformation
Answer: c) Hybridization

Which of the following is a technique used to study the interactions between proteins and small molecules in a high-throughput manner?
a) PCR
b) ELISA
c) Chemical proteomics
d) Western blotting
Answer: c) Chemical proteomics

What is the term for a technique used to determine the presence and quantity of specific proteins in a sample based on their migration in a gel under the influence of an electric field and subsequent transfer to a membrane?
a) PCR
b) ELISA
c) Western blotting
d) Southern blotting
Answer: c) Western blotting

 

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