Chemistry MCQs

Chromatography Techniques MCQs with Answers

Which chromatography technique relies on the differential affinity of components in a mixture between a stationary phase and a mobile phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their molecular size and shape?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Size exclusion chromatography (SEC)
d) Affinity chromatography (AC)
Answer: c) Size exclusion chromatography (SEC)

Which chromatography technique is commonly used for the separation of volatile compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: b) Gas chromatography (GC)

Which chromatography technique is based on the principle of partitioning between a stationary liquid phase and a mobile gas phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: b) Gas chromatography (GC)

Which chromatography technique uses a solid stationary phase and a liquid mobile phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: a) Thin-layer chromatography (TLC)

Which chromatography technique is used for the separation of charged species based on their interaction with an ion-exchange resin?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: d) Ion chromatography (IC)

Which chromatography technique is commonly used for the separation of amino acids and peptides?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Affinity chromatography (AC)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique utilizes the specific interaction between a target molecule and a ligand immobilized on a stationary phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Affinity chromatography (AC)
Answer: d) Affinity chromatography (AC)

Which chromatography technique is commonly used for the separation of enantiomers?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Chiral chromatography
Answer: d) Chiral chromatography

Which chromatography technique utilizes a solid stationary phase and a liquid mobile phase pumped at high pressure?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique is based on the selective adsorption of compounds onto a solid stationary phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Solid-phase extraction (SPE)
Answer: d) Solid-phase extraction (SPE)

Which chromatography technique is commonly used for the purification of proteins?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Size exclusion chromatography (SEC)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their affinity for a hydrophobic stationary phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Reverse-phase chromatography
Answer: d) Reverse-phase chromatography

Which chromatography technique is commonly used for the separation of nucleic acids?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Gel electrophoresis
Answer: d) Gel electrophoresis

Which chromatography technique separates compounds based on their interaction with a mobile phase that flows through a packed column?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique is commonly used for the separation of complex mixtures and the analysis of biomolecules?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a stationary phase coated on a solid support?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Solid-phase microextraction (SPME)
Answer: d) Solid-phase microextraction (SPME)

Which chromatography technique is commonly used for the separation of complex mixtures and the analysis of volatile compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Supercritical fluid chromatography (SFC)
Answer: b) Gas chromatography (GC)

Which chromatography technique separates compounds based on their affinity for a specific ligand immobilized on a stationary phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Affinity chromatography (AC)
Answer: d) Affinity chromatography (AC)

Which chromatography technique separates compounds based on their interaction with a mobile phase containing charged particles?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: d) Ion chromatography (IC)

Which chromatography technique is commonly used for the separation of nonpolar and moderately polar compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Normal-phase chromatography
d) Reverse-phase chromatography
Answer: c) Normal-phase chromatography

Which chromatography technique is commonly used for the separation of polar and moderately polar compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Normal-phase chromatography
d) Reverse-phase chromatography
Answer: d) Reverse-phase chromatography

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of a polar solid support and a nonpolar mobile phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Normal-phase chromatography
d) Reverse-phase chromatography
Answer: c) Normal-phase chromatography

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of a nonpolar solid support and a polar mobile phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Normal-phase chromatography
d) Reverse-phase chromatography
Answer: d) Reverse-phase chromatography

Which chromatography technique is commonly used for the separation of complex mixtures and the analysis of organic compounds in environmental samples?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Solid-phase microextraction (SPME)
Answer: b) Gas chromatography (GC)

Which chromatography technique separates compounds based on their interaction with a mobile phase consisting of a supercritical fluid?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Supercritical fluid chromatography (SFC)
Answer: d) Supercritical fluid chromatography (SFC)

Which chromatography technique is commonly used for the separation of polar and hydrophilic compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Hydrophilic interaction chromatography (HILIC)
Answer: d) Hydrophilic interaction chromatography (HILIC)

Which chromatography technique is commonly used for the separation of nonpolar and hydrophobic compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Reverse-phase chromatography
Answer: d) Reverse-phase chromatography

Which chromatography technique is commonly used for the separation of carbohydrates and sugars?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Affinity chromatography (AC)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique is commonly used for the separation and analysis of amino acids?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique is commonly used for the separation and analysis of lipids?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of a hydrophilic solid support and a hydrophobic mobile phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Hydrophilic interaction chromatography (HILIC)
d) Reverse-phase chromatography
Answer: c) Hydrophilic interaction chromatography (HILIC)

Which chromatography technique is commonly used for the separation and analysis of natural products?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique is commonly used for the purification of synthetic compounds and drugs?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Preparative chromatography
Answer: d) Preparative chromatography

Which chromatography technique separates compounds based on their affinity for a specific antibody immobilized on a stationary phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Affinity chromatography (AC)
Answer: d) Affinity chromatography (AC)

Which chromatography technique is commonly used for the separation and analysis of environmental pollutants?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Solid-phase extraction (SPE)
Answer: b) Gas chromatography (GC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of a chiral selector?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Chiral chromatography
d) Reverse-phase chromatography
Answer: c) Chiral chromatography

Which chromatography technique is commonly used for the separation and analysis of natural pigments?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a mobile phase consisting of a polar solvent and a nonpolar solvent?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Normal-phase chromatography
d) Reverse-phase chromatography
Answer: c) Normal-phase chromatography

Which chromatography technique is commonly used for the separation and analysis of pharmaceutical compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of an immobilized antibody?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Affinity chromatography (AC)
d) Solid-phase microextraction (SPME)
Answer: c) Affinity chromatography (AC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of a porous solid support?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Flash chromatography
Answer: d) Flash chromatography

Which chromatography technique is commonly used for the separation and analysis of pesticides?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of a polar solid support and a nonpolar mobile phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Normal-phase chromatography
d) Reverse-phase chromatography
Answer: d) Reverse-phase chromatography

Which chromatography technique is commonly used for the separation and analysis of carbohydrates and sugars?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a mobile phase consisting of a supercritical fluid?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Supercritical fluid chromatography (SFC)
Answer: d) Supercritical fluid chromatography (SFC)

Which chromatography technique is commonly used for the separation and analysis of natural products?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of a hydrophilic solid support and a hydrophobic mobile phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Hydrophilic interaction chromatography (HILIC)
d) Reverse-phase chromatography
Answer: c) Hydrophilic interaction chromatography (HILIC)

Which chromatography technique is commonly used for the separation and analysis of volatile compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Gas chromatography-mass spectrometry (GC-MS)
Answer: b) Gas chromatography (GC)

Which chromatography technique is commonly used for the separation and analysis of amino acids?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Ion chromatography (IC)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of an immobilized antibody?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Affinity chromatography (AC)
d) Solid-phase microextraction (SPME)
Answer: c) Affinity chromatography (AC)

Which chromatography technique is commonly used for the separation and analysis of environmental pollutants?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Solid-phase extraction (SPE)
Answer: b) Gas chromatography (GC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of a chiral selector?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Chiral chromatography
d) Reverse-phase chromatography
Answer: c) Chiral chromatography

Which chromatography technique is commonly used for the separation and analysis of natural pigments?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their affinity for a specific ligand immobilized on a stationary phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Affinity chromatography (AC)
Answer: d) Affinity chromatography (AC)

Which chromatography technique is commonly used for the separation and analysis of pharmaceutical compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of an immobilized antibody?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Affinity chromatography (AC)
d) Solid-phase microextraction (SPME)
Answer: c) Affinity chromatography (AC)

Which chromatography technique is commonly used for the separation and analysis of environmental pollutants?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Solid-phase extraction (SPE)
Answer: b) Gas chromatography (GC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of a chiral selector?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Chiral chromatography
d) Reverse-phase chromatography
Answer: c) Chiral chromatography

Which chromatography technique is commonly used for the separation and analysis of natural pigments?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their affinity for a specific ligand immobilized on a stationary phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Affinity chromatography (AC)
Answer: d) Affinity chromatography (AC)

Which chromatography technique is commonly used for the separation and analysis of pharmaceutical compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of an immobilized antibody?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Affinity chromatography (AC)
d) Solid-phase microextraction (SPME)
Answer: c) Affinity chromatography (AC)

Which chromatography technique is commonly used for the separation and analysis of environmental pollutants?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Solid-phase extraction (SPE)
Answer: b) Gas chromatography (GC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of a chiral selector?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Chiral chromatography
d) Reverse-phase chromatography
Answer: c) Chiral chromatography

Which chromatography technique is commonly used for the separation and analysis of natural pigments?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their affinity for a specific ligand immobilized on a stationary phase?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Affinity chromatography (AC)
Answer: d) Affinity chromatography (AC)

Which chromatography technique is commonly used for the separation and analysis of pharmaceutical compounds?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Liquid chromatography-mass spectrometry (LC-MS)
Answer: c) High-performance liquid chromatography (HPLC)

Which chromatography technique separates compounds based on their interaction with a stationary phase consisting of an immobilized antibody?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) Affinity chromatography (AC)
d) Solid-phase microextraction (SPME)
Answer: c) Affinity chromatography (AC)

Which chromatography technique is commonly used for the separation and analysis of environmental pollutants?
a) Thin-layer chromatography (TLC)
b) Gas chromatography (GC)
c) High-performance liquid chromatography (HPLC)
d) Solid-phase extraction (SPE)
Answer: b) Gas chromatography (GC)

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